224 TETRACYCLINE-INDUCIBLE GENE EXPRESSION WITH RETROVIRUS VECTOR
نویسندگان
چکیده
منابع مشابه
Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector
DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with polyethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and...
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The recent development of tetracycline (tet)-regulated transactivation systems for inducible gene expression has dramatically enhanced the tools available for the temporal and quantitative control of exogenous genes in mammalian cells and transgenic mice and plants. Such systems have applications in many areas of biology and medicine, including the study of gene regulation and function in devel...
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ژورنال
عنوان ژورنال: Reproduction, Fertility and Development
سال: 2005
ISSN: 1031-3613
DOI: 10.1071/rdv17n2ab224